6ZRZ
Crystal structure of 5-dimethylallyl tryptophan synthase from Streptomyces coelicolor in complex with DMASPP and Trp
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-07 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00002 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.658, 70.147, 91.082 |
| Unit cell angles | 90.00, 92.72, 90.00 |
Refinement procedure
| Resolution | 45.490 - 1.696 |
| Rwork | 0.181 |
| R-free | 0.21890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5inj |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.455 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (7.1.001) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.500 | 1.800 |
| High resolution limit [Å] | 1.696 | 1.696 |
| Rmeas | 0.065 | 1.000 |
| Number of reflections | 62994 | 10011 |
| <I/σ(I)> | 15.4 | |
| Completeness [%] | 97.0 | |
| Redundancy | 4.5 | |
| CC(1/2) | 0.990 | 0.590 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 150 mM Bis-Tris 20% PEG 3350 |
