6ZRX
Crystal structure of 6-dimethylallyltryptophan synthase from Micromonospora olivasterospora in complex with DMASPP and Trp
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00001 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.021, 98.471, 89.185 |
| Unit cell angles | 90.00, 107.49, 90.00 |
Refinement procedure
| Resolution | 39.673 - 1.700 |
| Rwork | 0.202 |
| R-free | 0.23020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5inj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.246 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (7.1.001) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.673 | 1.790 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmeas | 0.090 | 0.680 |
| Number of reflections | 143483 | 92053 |
| <I/σ(I)> | 11.3 | 2.1 |
| Completeness [%] | 98.5 | 98.5 |
| Redundancy | 4 | 4 |
| CC(1/2) | 0.990 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 200 mM Calcium acetate, 26% PEG 3350 |
