6ZRX
Crystal structure of 6-dimethylallyltryptophan synthase from Micromonospora olivasterospora in complex with DMASPP and Trp
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00001 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.021, 98.471, 89.185 |
Unit cell angles | 90.00, 107.49, 90.00 |
Refinement procedure
Resolution | 39.673 - 1.700 |
Rwork | 0.202 |
R-free | 0.23020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5inj |
RMSD bond length | 0.007 |
RMSD bond angle | 1.246 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (7.1.001) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.673 | 1.790 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmeas | 0.090 | 0.680 |
Number of reflections | 143483 | 92053 |
<I/σ(I)> | 11.3 | 2.1 |
Completeness [%] | 98.5 | 98.5 |
Redundancy | 4 | 4 |
CC(1/2) | 0.990 | 0.820 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 200 mM Calcium acetate, 26% PEG 3350 |