6ZRW
Crystal structure of the fungal lectin CML1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0721 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.497, 73.626, 111.956 |
| Unit cell angles | 90.00, 101.03, 90.00 |
Refinement procedure
| Resolution | 61.170 - 1.350 |
| R-factor | 0.1473 |
| Rwork | 0.146 |
| R-free | 0.17600 |
| Structure solution method | SAD |
| Data scaling software | XDS |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.170 | 1.430 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 303404 | 47202 |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 98.9 | |
| Redundancy | 7.4 | |
| CC(1/2) | 1.000 | 0.634 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dehydrate pH 5.6, 30% polyethylene glycol 4000 |
