6ZRS
Racemic compound of RNA duplexes.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.283 |
Spacegroup name | P 1 |
Unit cell lengths | 20.970, 26.430, 38.680 |
Unit cell angles | 105.84, 96.66, 92.03 |
Refinement procedure
Resolution | 36.893 - 1.520 |
Rwork | 0.187 |
R-free | 0.23520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6zq9 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.746 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.000 | 1.610 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.069 | 0.566 |
Rmeas | 0.081 | 0.664 |
Number of reflections | 10765 | 1466 |
<I/σ(I)> | 9.49 | 1.78 |
Completeness [%] | 88.2 | 74.1 |
Redundancy | 3.55 | 3.5 |
CC(1/2) | 0.998 | 0.889 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M zinc acetate, 0.1 M cacodylate buffer, 18% w/v polyehylene glycol 8000 |