6ZRC

Structure of the human RBAP48 in complex with a macrocyclic peptide cyclized via a xylene linker attached to two cysteines

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.99998
Spacegroup name	C 1 2 1
Unit cell lengths	71.960, 96.325, 149.300
Unit cell angles	90.00, 90.01, 90.00

Refinement procedure

Resolution	48.160 - 2.600
R-factor	0.2642
Rwork	0.263
R-free	0.29280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pbz
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18rc7_3834)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.160	48.160	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.111	0.029	1.264
Rmeas	0.120	0.032	1.366
Total number of observations	212828
Number of reflections	31297	370	2303
<I/σ(I)>	9.91	44.9	1.11
Completeness [%]	99.5	98.4	99.8
Redundancy	6.8	6.235	7.016
CC(1/2)	0.999	0.999	0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.1	293	20% PEG3350 0.15M DL-malic acid pH 7.1