6ZR4
Crystal structure of tetrameric fibrinogen-like recognition domain of FIBCD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 4 |
| Unit cell lengths | 118.349, 118.349, 44.089 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.830 - 2.000 |
| R-factor | 0.1665 |
| Rwork | 0.166 |
| R-free | 0.18420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 4M7H |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.304 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.6.2) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 83.830 | 83.690 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.117 | 0.059 | 0.389 |
| Rmeas | 0.135 | 0.069 | 0.452 |
| Rpim | 0.067 | 0.035 | 0.225 |
| Total number of observations | 1784 | 10903 | |
| Number of reflections | 41341 | 514 | 3000 |
| <I/σ(I)> | 8.3 | 14.7 | 3.7 |
| Completeness [%] | 99.1 | 99.3 | 99.3 |
| Redundancy | 3.7 | 3.5 | 3.6 |
| CC(1/2) | 0.992 | 0.986 | 0.846 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.7 M Ammonium Sulphate, 9% Dioxane, 0.1 M MES pH 6.5 |
