6ZR1

Racemic compound of RNA duplexes.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.283
Spacegroup name	P 1
Unit cell lengths	21.130, 26.760, 38.820
Unit cell angles	106.09, 97.19, 91.42

Refinement procedure

Resolution	36.945 - 1.528
Rwork	0.203
R-free	0.25270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zq9
RMSD bond length	0.010
RMSD bond angle	1.737
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.000	1.620
High resolution limit [Å]	1.528	1.528
Rmerge	0.086	0.557
Rmeas	0.096	0.618
Number of reflections	10955	1494
<I/σ(I)>	9.4	2.03
Completeness [%]	89.2	74.7
Redundancy	5.3	5.1
CC(1/2)	0.997	0.946

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M zinc aceate, 0.1 M cacodylate buffer, 18% w/v polyethylene glycol 8000