6ZQX
Crystal structure of tetrameric fibrinogen-like recognition domain of FIBCD1 with N,N'-diacetyl chitobiose ligand bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 4 |
| Unit cell lengths | 118.670, 118.670, 44.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.410 - 1.840 |
| R-factor | 0.162 |
| Rwork | 0.161 |
| R-free | 0.17630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 4M7H |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.431 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 59.410 | 59.330 | 1.880 |
| High resolution limit [Å] | 1.840 | 9.010 | 1.840 |
| Rmerge | 0.067 | 0.024 | 0.328 |
| Rmeas | 0.083 | 0.030 | 0.403 |
| Rpim | 0.047 | 0.018 | 0.231 |
| Total number of observations | 1302 | 9208 | |
| Number of reflections | 53600 | 496 | 3248 |
| <I/σ(I)> | 9.8 | 22.3 | 2.9 |
| Completeness [%] | 99.5 | 98 | 99.7 |
| Redundancy | 2.8 | 2.6 | 2.8 |
| CC(1/2) | 0.997 | 0.998 | 0.861 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.6 M Ammonium Sulphate, 8% Dioxane, 0.1 M MES pH 6.5 |
