6ZQP

Structure of the Pmt2-MIR domain with bound ligands

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.072
Spacegroup name	P 41 3 2
Unit cell lengths	139.640, 139.640, 139.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.472 - 1.600
R-factor	0.1411
Rwork	0.141
R-free	0.15710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mal
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.472	1.660
High resolution limit [Å]	1.600	1.600
Number of reflections	61507	6045
<I/σ(I)>	13.45
Completeness [%]	99.8
Redundancy	9.4
CC(1/2)	0.998	0.527

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.7 M AS, 1.7% (v/v) PEG400, 0.085 M HEPES pH 7.5, 15% (v/v) glycerol