6ZPX

Structure of Unliganded MgGH51 a-L-Arabinofuranosidase Crystal Type 1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-14
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	59.980, 65.801, 193.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.362 - 1.300
Rwork	0.178
R-free	0.21740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zps
RMSD bond length	0.016
RMSD bond angle	1.899
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.280	1.320
High resolution limit [Å]	1.300	1.300
Rpim	0.035	0.422
Number of reflections	188207	9226
<I/σ(I)>	6.9
Completeness [%]	100.0	99.7
Redundancy	7.8	6
CC(1/2)	0.998	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	10 mg/mL MgGH51 in 10 mM NaOAc, pH 5.5, 100 mM NaCl mixed 2:1 with 20% PEG 3350, 0.1 M Bis-Tris-HCl, pH 6.5, 0.2 M NaNO3