6ZPE
Nonstructural protein 10 (nsp10) from SARS CoV-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 106.624, 106.624, 106.624 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.529 - 1.580 |
| R-factor | 0.1519 |
| Rwork | 0.151 |
| R-free | 0.15980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6zct |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.091 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 75.390 | 75.390 | 1.610 |
| High resolution limit [Å] | 1.580 | 8.650 | 1.580 |
| Rmerge | 0.044 | 0.045 | 1.217 |
| Rmeas | 0.047 | 0.049 | 1.283 |
| Rpim | 0.015 | 0.018 | 0.402 |
| Total number of observations | 1237 | 14080 | |
| Number of reflections | 27706 | 185 | 1391 |
| <I/σ(I)> | 24.1 | 61.2 | 2 |
| Completeness [%] | 99.9 | 95.8 | 100 |
| Redundancy | 10 | 6.7 | 10.1 |
| CC(1/2) | 0.999 | 0.995 | 0.709 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M Bis-Tris pH 5.5, 2.17 M NaCl |
