6ZPB
Cyanophage S-2L HD phosphohydrolase (DatZ) bound to dA and two catalytic Co2+ ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033202 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 141.827, 141.827, 53.725 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.436 - 1.721 |
| R-factor | 0.147191339442 |
| Rwork | 0.146 |
| R-free | 0.17021 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.282 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXCD |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.440 | 1.770 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.083 | 0.307 |
| Rmeas | 0.086 | 0.338 |
| Rpim | 0.022 | 0.137 |
| Number of reflections | 21613 | 1400 |
| <I/σ(I)> | 25 | 5.1 |
| Completeness [%] | 98.4 | 88 |
| Redundancy | 14.2 | 5.7 |
| CC(1/2) | 0.999 | 0.939 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 1.5 M LiSO4; 100 mM HEPES; 10 mM CoCl2 |
