6ZP9

Cyanophage S-2L Primase-Polymerase (PrimPol), AEP domain (PP-N190)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980097
Spacegroup name	P 1 21 1
Unit cell lengths	59.177, 47.686, 66.155
Unit cell angles	90.00, 97.11, 90.00

Refinement procedure

Resolution	41.300 - 1.500
R-factor	0.166003042424
Rwork	0.166
R-free	0.17274
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.854
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXCD
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.690	1.540
High resolution limit [Å]	1.500	1.500
Rmerge	0.102	1.399
Rmeas	0.110	1.511
Rpim	0.041	0.563
Number of reflections	56799	4110
<I/σ(I)>	11.8	1.4
Completeness [%]	96.8	94.6
Redundancy	7.1	7
CC(1/2)	0.999	0.555

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277.15	100 mM CaCl2; 20% w/v PEG 8k (40%); 5% v/v isopropanol (100%); 100 mM MES pH 6