6ZP9
Cyanophage S-2L Primase-Polymerase (PrimPol), AEP domain (PP-N190)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-15 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980097 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.177, 47.686, 66.155 |
| Unit cell angles | 90.00, 97.11, 90.00 |
Refinement procedure
| Resolution | 41.300 - 1.500 |
| R-factor | 0.166003042424 |
| Rwork | 0.166 |
| R-free | 0.17274 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.854 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXCD |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.690 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.102 | 1.399 |
| Rmeas | 0.110 | 1.511 |
| Rpim | 0.041 | 0.563 |
| Number of reflections | 56799 | 4110 |
| <I/σ(I)> | 11.8 | 1.4 |
| Completeness [%] | 96.8 | 94.6 |
| Redundancy | 7.1 | 7 |
| CC(1/2) | 0.999 | 0.555 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277.15 | 100 mM CaCl2; 20% w/v PEG 8k (40%); 5% v/v isopropanol (100%); 100 mM MES pH 6 |
