6ZNX
Ribokinase from Thermus Species
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.210, 155.550, 83.040 |
| Unit cell angles | 90.00, 98.20, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.2295 |
| Rwork | 0.227 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xda |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.616 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.077 | 0.140 |
| Number of reflections | 41928 | 6150 |
| <I/σ(I)> | 5.2958 | 4.47 |
| Completeness [%] | 96.9 | 97.73 |
| Redundancy | 3.18 | 3.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 293 | PEG |
