6ZNB

PHAGE SAM LYASE IN APO STATE

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0722
Spacegroup name	F 41 3 2
Unit cell lengths	158.477, 158.477, 158.477
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.620 - 2.800
R-factor	0.2345
Rwork	0.232
R-free	0.27370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zm9
RMSD bond length	0.003
RMSD bond angle	0.460
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.620	2.950
High resolution limit [Å]	2.800	2.800
Rmeas	0.125	0.939
Number of reflections	4409	634
<I/σ(I)>	9.6	1.8
Completeness [%]	97.7	98.5
Redundancy	5.2	5.3
CC(1/2)	0.993	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.5 M ammonium phosphate