6ZN7
MaeB malic enzyme domain apoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97633 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.817, 92.192, 95.796 |
Unit cell angles | 90.00, 91.13, 90.00 |
Refinement procedure
Resolution | 95.777 - 1.670 |
R-factor | 0.1813 |
Rwork | 0.179 |
R-free | 0.21770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cee |
RMSD bond length | 0.007 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.780 | 1.700 |
High resolution limit [Å] | 1.670 | 1.670 |
Number of reflections | 96099 | 4736 |
<I/σ(I)> | 6.87 | |
Completeness [%] | 99.9 | |
Redundancy | 6.52 | |
CC(1/2) | 0.990 | 0.470 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 9.3 | 289 | 0.1M Bicine pH 9.3 25% PEG smear medium |