6ZMX
Crystal structure of hemoglobin from turkey (Meleagiris gallopova) crystallized in orthorhombic form at 1.4 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-21 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.760, 82.140, 90.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.835 - 1.389 |
| R-factor | 0.1983 |
| Rwork | 0.197 |
| R-free | 0.22330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1faw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.905 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.835 | 1.413 |
| High resolution limit [Å] | 1.389 | 1.389 |
| Rmerge | 0.084 | |
| Rmeas | 0.096 | |
| Number of reflections | 118908 | 5867 |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 99.0 | 98.1 |
| Redundancy | 4.4 | 4.4 |
| CC(1/2) | 0.997 | 0.514 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 40% of PEG 3350 in 50mM phosphate buffer at pH7.2 |
