6ZMV

Structure of muramidase from Trichobolus zukalii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 1
Unit cell lengths	39.398, 48.582, 55.737
Unit cell angles	97.08, 99.53, 98.15

Refinement procedure

Resolution	27.170 - 1.400
Rwork	0.104
R-free	0.14270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x8r
RMSD bond length	0.016
RMSD bond angle	1.909
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.187	1.436
High resolution limit [Å]	1.400	1.400
Number of reflections	73721	2699
<I/σ(I)>	15.8
Completeness [%]	93.8
Redundancy	2.1
CC(1/2)	0.992	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	lithium sulphate, ammonium sulphate, sodium citrate