6ZMG

PHAGE SAM LYASE IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9184
Spacegroup name	F 41 3 2
Unit cell lengths	154.473, 154.473, 154.473
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.590 - 1.480
R-factor	0.1423
Rwork	0.141
R-free	0.17170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zm9
RMSD bond length	0.009
RMSD bond angle	1.105
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.610	1.570
High resolution limit [Å]	1.480	1.480
Rmerge	0.123	1.110
Number of reflections	26719	4522
<I/σ(I)>	32.44	2.04
Completeness [%]	99.7	97
Redundancy	95	24.9
CC(1/2)	1.000	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.6 M AMMONIUM PHOSPHATE