6ZMB
Structure of the native tRNA-Monooxygenase enzyme MiaE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9753 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.797, 50.929, 76.258 |
| Unit cell angles | 90.00, 91.00, 90.00 |
Refinement procedure
| Resolution | 46.752 - 1.700 |
| R-factor | 0.1897 |
| Rwork | 0.188 |
| R-free | 0.21660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2itb |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmeas | 0.040 | 0.846 |
| Number of reflections | 48290 | 7733 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 97.5 | 97.7 |
| Redundancy | 2.8 | 2.8 |
| CC(1/2) | 0.999 | 0.845 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | MiaE: 20 mg/ml in 100 mM HEPES, pH 7.5, 30mM NaCl reservoir solution: 0.5M CaCl2, 42% PEG 6k, 2M Tris-Cl pH 8 |
