6ZM8
Structure of muramidase from Acremonium alcalophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.65 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.087, 77.272, 35.728 |
| Unit cell angles | 90.00, 104.19, 90.00 |
Refinement procedure
| Resolution | 38.636 - 0.780 |
| Rwork | 0.122 |
| R-free | 0.13370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x8r |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.980 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.640 | 0.790 |
| High resolution limit [Å] | 0.780 | 0.780 |
| Number of reflections | 201033 | 8829 |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 99.1 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.979 | 0.356 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M NaCl, 0.1M phosphate/citrate pH 4.2, 20% W/v PEG 8000 |
