6ZM2

Crystal structure of the DEAH-box ATPase Prp2 in complex with ADP-BeF3 and ssRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979300
Spacegroup name	P 21 21 21
Unit cell lengths	48.660, 100.410, 140.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.780 - 2.100
R-factor	0.1875
Rwork	0.185
R-free	0.23310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fac
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	81.780	81.780	2.200
High resolution limit [Å]	2.100	50.000	2.100
Rmerge	0.126	3.706	1.279
Rmeas	0.131	5.242	1.326
Total number of observations	561018
Number of reflections	41019	3	5228
<I/σ(I)>	14.88	0.91	2.21
Completeness [%]	99.5	75	99
Redundancy	13.677	2	13.901
CC(1/2)	0.999	1.000	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	100mM MOPS Na-HEPES, 8% PEG20000, 22% PEGMME 550, 20mM 1,6-hexanediol, 20mM 1-butanol, 20mM 1,2-propanediol, 20mM 2-propanol, 20mM 1,4-butandiol, 20mM 1,3-propanediol