6ZL4
the structure of glutamate transporter homologue GltTk in complex with the photo switchable compound (cis)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 117.210, 117.210, 309.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.225 - 3.000 |
| R-factor | 0.2562 |
| Rwork | 0.255 |
| R-free | 0.28760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e9s |
| Data reduction software | XSCALE |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.225 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 50233 | 3663 |
| <I/σ(I)> | 10.12 | 0.31 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 15.3 | |
| CC(1/2) | 0.998 | 0.124 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 277.15 | 23% PEG 400, 100 mM sodium acetate, 50 mM NaCl, 20 mM MgCl2 |
