6ZK5
Plant nucleoside hydrolase - ZmNRh3 enzyme in complex with forodesine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-27 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.978 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.540, 79.270, 85.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.610 - 1.900 |
R-factor | 0.171 |
Rwork | 0.170 |
R-free | 0.19300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kpo |
RMSD bond length | 0.010 |
RMSD bond angle | 1.040 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.610 | 2.010 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.149 | |
Number of reflections | 42550 | 6773 |
<I/σ(I)> | 13.7 | |
Completeness [%] | 99.8 | |
Redundancy | 13.3 | |
CC(1/2) | 0.999 | 0.572 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | PEG |