6ZK1

Plant nucleoside hydrolase - ZmNRh2b enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.978
Spacegroup name	P 1 21 1
Unit cell lengths	47.790, 148.650, 88.580
Unit cell angles	90.00, 102.24, 90.00

Refinement procedure

Resolution	28.990 - 1.990
R-factor	0.176
Rwork	0.175
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kpo
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.120
High resolution limit [Å]	1.990	1.990
Rmerge	0.072
Number of reflections	81500	12219
<I/σ(I)>	11.2
Completeness [%]	98.3
Redundancy	3.7
CC(1/2)	0.998	0.705

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	PEG4000, Tris