6ZJT
Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB mutant E441Q in complex with lactulose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-25 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 138.974, 138.974, 127.699 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.793 - 1.970 |
R-factor | 0.1952 |
Rwork | 0.195 |
R-free | 0.22560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6etz |
RMSD bond length | 0.009 |
RMSD bond angle | 1.081 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.793 | 43.793 | 2.090 |
High resolution limit [Å] | 1.970 | 5.860 | 1.970 |
Rmerge | 0.071 | 0.031 | 1.592 |
Rmeas | 0.079 | 0.034 | 1.753 |
Total number of observations | 562868 | ||
Number of reflections | 100446 | 3972 | 15949 |
<I/σ(I)> | 12.14 | 44.9 | 0.8 |
Completeness [%] | 99.6 | 97.9 | 98.9 |
Redundancy | 5.604 | 5.094 | 5.692 |
CC(1/2) | 0.999 | 0.999 | 0.655 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 37% TacsimateTM pH 8.0 |