6ZJS
Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB mutant E441Q in complex with galactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-11 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9814 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 138.378, 138.378, 127.807 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.944 - 1.500 |
R-factor | 0.1435 |
Rwork | 0.143 |
R-free | 0.16110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6etz |
RMSD bond length | 0.008 |
RMSD bond angle | 0.996 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.944 | 46.944 | 1.590 |
High resolution limit [Å] | 1.499 | 4.480 | 1.500 |
Rmerge | 0.089 | 0.038 | 3.368 |
Rmeas | 0.093 | 0.040 | 3.546 |
Number of reflections | 224751 | 8815 | 36003 |
<I/σ(I)> | 13.44 | 56.27 | 0.58 |
Completeness [%] | 99.9 | 99.9 | 99.6 |
Redundancy | 10.141 | 9.868 | 10.174 |
CC(1/2) | 0.999 | 0.999 | 0.293 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 37% TacsimateTM pH 8.0 |