6ZJQ

Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB mutant E517Q in complex with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9814
Spacegroup name	P 31 2 1
Unit cell lengths	138.608, 138.608, 127.656
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.724 - 1.700
R-factor	0.165
Rwork	0.165
R-free	0.19590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6etz
RMSD bond length	0.009
RMSD bond angle	0.977
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.724	43.724	1.800
High resolution limit [Å]	1.699	5.070	1.700
Rmerge	0.129	0.028	3.621
Rmeas	0.136	0.030	3.809
Number of reflections	155102	6144	24686
<I/σ(I)>	13.47	65.42	0.63
Completeness [%]	99.8	99.8	98.9
Redundancy	10.186	9.707	10.387
CC(1/2)	0.999	0.999	0.297

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	37% TacsimateTM pH 8.0