6ZJP

Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB mutant E517Q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9814
Spacegroup name	P 31 2 1
Unit cell lengths	137.996, 137.996, 127.219
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.552 - 1.850
R-factor	0.162
Rwork	0.162
R-free	0.18900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6etz
RMSD bond length	0.004
RMSD bond angle	0.744
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.552	43.552	1.960
High resolution limit [Å]	1.849	5.510	1.850
Rmerge	0.067	0.033	0.729
Rmeas	0.075	0.037	0.817
Number of reflections	118428	4594	18923
<I/σ(I)>	12.39	38.12	1.52
Completeness [%]	99.2	96.7	98.6
Redundancy	4.944	4.942	4.897
CC(1/2)	0.998	0.998	0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	40% Tacsimate TM pH 8.0