6ZJ0

CRYSTAL STRUCTURE OF HRAS-G12D IN COMPLEX WITH GCP AND COMPOUND 18

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-07
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	P 3 2 1
Unit cell lengths	93.075, 93.075, 55.256
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	80.610 - 1.763
R-factor	0.1999
Rwork	0.198
R-free	0.22760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3l8z
RMSD bond length	0.008
RMSD bond angle	0.930
Data reduction software	autoPROC
Data scaling software	autoPROC ((Version 1.1.7))
Phasing software	PHASER
Refinement software	BUSTER (2.11.7 (17-DEC-2019))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.610	1.905
High resolution limit [Å]	1.763	1.764
Rmerge	0.054
Number of reflections	22394	1119
<I/σ(I)>	27.9	1.3
Completeness [%]	81.4	20
Redundancy	14.9	14.7
CC(1/2)		0.524

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		278	30% PEG400, 200mM MgCl2, 100mM TRIS PH=8,5