6ZIX
Structure of RcsB from Salmonella enterica serovar Typhimurium bound to promoter P1flhDC in the presence of phosphomimetic BeF3-
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | H 3 |
| Unit cell lengths | 183.220, 183.220, 84.143 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.790 - 3.400 |
| R-factor | 0.2407 |
| Rwork | 0.239 |
| R-free | 0.27350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o8z |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 74.340 | 74.340 | 3.670 |
| High resolution limit [Å] | 3.400 | 9.000 | 3.400 |
| Rmerge | 0.053 | 0.035 | 0.473 |
| Rmeas | 0.060 | 0.039 | 0.532 |
| Rpim | 0.028 | 0.018 | 0.241 |
| Total number of observations | 69020 | 3512 | 14473 |
| Number of reflections | 14477 | 788 | 2999 |
| <I/σ(I)> | 11.8 | 35.8 | 3.1 |
| Completeness [%] | 99.9 | 99.5 | 99.9 |
| Redundancy | 4.8 | 4.5 | 4.8 |
| CC(1/2) | 0.998 | 0.996 | 0.963 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 1.5M ammonium sulfate, 1.6% glycerol and 0.8M lithium sulfate |
