Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

6ZI9

Ultrafast Structural Response to Charge Redistribution Within a Photosynthetic Reaction Centre - 300 ps (b) structure

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeFREE ELECTRON LASER
Source detailsSLAC LCLS BEAMLINE CXI
Synchrotron siteSLAC LCLS
BeamlineCXI
Temperature [K]293
Detector technologyPIXEL
Collection date2016-06-13
DetectorCS-PAD CXI-1
Wavelength(s)1.3
Spacegroup nameP 43 21 2
Unit cell lengths226.500, 226.500, 113.900
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution34.310 - 2.800
R-factor0.1672
Rwork0.166
R-free0.18980
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2i5n
RMSD bond length0.008
RMSD bond angle2.092
Data reduction softwareCrystFEL (0.6.3)
Data scaling softwareCrystFEL (0.6.3)
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0158)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]35.8002.830
High resolution limit [Å]2.8002.800
Number of reflections750914961
<I/σ(I)>12.140.88
Completeness [%]100.0
Redundancy2042
CC(1/2)0.9970.345
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP277Macrocrystal growth: 10 mg/ml protein, 3.6 M ammonium sulphate, 6 % w/v heptane-1,2,3-triol, 20 mM KH203/K2H03 pH 6.8, 0.1 % LDAO 20 ul sitting drop, 10 protein : 10 precipitant Microcrystals: 8.5 mg/ml protein 18.5 ul sitting drop, 10 protein : 7.5 precipitant : 1 seed stock

229380

PDB entries from 2024-12-25

PDB statisticsPDBj update infoContact PDBjnumon