6ZFF

Pyrococcus furiosus Rad50 coiled coils in rod configuration

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-04
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.280980
Spacegroup name	I 41
Unit cell lengths	128.300, 128.300, 74.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.370 - 3.000
R-factor	0.254
Rwork	0.250
R-free	0.29470
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.319
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CRANK2
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.370	3.108
High resolution limit [Å]	3.000	3.001
Number of reflections	12199	1216
<I/σ(I)>	18.71
Completeness [%]	98.4
Redundancy	13.5
CC(1/2)	0.999	0.937

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	2.25 M ammonium sulfate and 50 mM Tris/HCl pH 8