6ZFD
14-3-3 zeta bound to the phosphorylated 18E6 C-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-09 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.350, 78.530, 90.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.140 - 1.900 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.20710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o02 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.826 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.200 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.166 | 2.050 |
| Number of reflections | 41285 | 2986 |
| <I/σ(I)> | 13.19 | 1.39 |
| Completeness [%] | 100.0 | |
| Redundancy | 13.3 | |
| CC(1/2) | 0.990 | 0.547 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 19% PEG4000, 0.1M cacodylate, 20%glycerol as a cryoprotectant |
