6ZEK
Crystal structure of mouse CSAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-23 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.940, 113.260, 113.370 |
| Unit cell angles | 90.00, 95.81, 90.00 |
Refinement procedure
| Resolution | 46.890 - 2.100 |
| R-factor | 0.1806 |
| Rwork | 0.179 |
| R-free | 0.23520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jis |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.864 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.151 | 1.411 |
| Number of reflections | 96877 | 6937 |
| <I/σ(I)> | 7.7 | 0.9 |
| Completeness [%] | 90.7 | 88.5 |
| Redundancy | 2.7 | 2.7 |
| CC(1/2) | 0.993 | 0.389 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.15 M KBr, 30% PEG 2000 MME |
