6ZE1
human NBD1 of CFTR in complex with nanobody G11a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 126.949, 126.949, 117.429 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 109.940 - 2.705 |
R-factor | 0.2098 |
Rwork | 0.207 |
R-free | 0.25760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gjs |
RMSD bond length | 0.008 |
RMSD bond angle | 0.980 |
Data reduction software | Aimless |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3 (20-MAY-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 109.940 | 3.081 |
High resolution limit [Å] | 2.705 | 2.705 |
Number of reflections | 7989 | 399 |
<I/σ(I)> | 8 | |
Completeness [%] | 93.3 | |
Redundancy | 12.2 | |
CC(1/2) | 0.996 | 0.647 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M Calcium chloride 0.1 M Tris 8.5 25 % w/v PEG 4000, RT. |