6ZDX

RIFIN variable region bound to LILRB1 ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97857
Spacegroup name	P 61 2 2
Unit cell lengths	134.507, 134.507, 277.468
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	89.210 - 3.000
R-factor	0.235
Rwork	0.234
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ewa 4ll9
RMSD bond length	0.011
RMSD bond angle	1.250
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	277.470	3.160
High resolution limit [Å]	3.000	3.000
Number of reflections	30613	4346
<I/σ(I)>	9.4
Completeness [%]	100.0
Redundancy	19.7
CC(1/2)	0.990	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	200 mM ammonium acetate, 100 mM HEPES pH 7.5, 25 % v/v isopropanol