6ZDT
Crystal structure of eukaryotic Fibrillarin with Nop56 N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.033198 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 69.004, 118.286, 48.828 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.830 - 1.710 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.20490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ipx |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.686 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.18rc4_3812) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.830 | 48.830 | 1.800 |
| High resolution limit [Å] | 1.710 | 5.400 | 1.710 |
| Rmerge | 0.075 | 0.021 | 1.016 |
| Rmeas | 0.078 | 0.022 | 1.057 |
| Rpim | 0.021 | 0.006 | 0.290 |
| Total number of observations | 582147 | 19171 | 83601 |
| Number of reflections | 44280 | 1558 | 6368 |
| <I/σ(I)> | 22.1 | 80 | 2.6 |
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 13.1 | 12.3 | 13.1 |
| CC(1/2) | 1.000 | 1.000 | 0.896 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 285 | 0.1 M LiCl, 0.1 M HEPES, 19 % PEG 400, Cryo: 10% 2,3-butanediol |
