6ZD6

Structure of apo telomerase from Candida Tropicalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980109
Spacegroup name	P 41 21 2
Unit cell lengths	65.695, 65.695, 387.615
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.770 - 2.640
R-factor	0.2222
Rwork	0.220
R-free	0.27250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zd1
RMSD bond length	0.003
RMSD bond angle	0.624
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.770	2.736
High resolution limit [Å]	2.640	2.642
Rmerge	0.177	2.347
Rmeas	0.183	2.432
Rpim	0.048	0.635
Number of reflections	47515	225
<I/σ(I)>	10.99	1.3
Completeness [%]	83.2	48.8
Redundancy	14.5	14.8
CC(1/2)	0.998	0.575

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.1	298	0.2 M sodium citrate tribasic dihydrate 0.1 M Bis-Tris propane (pH 6.1) 15% PEG3350