6ZD3
Crystal structure of YTHDC1 M438A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-17 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.710, 103.130, 42.310 |
| Unit cell angles | 90.00, 105.42, 90.00 |
Refinement procedure
| Resolution | 40.790 - 1.250 |
| R-factor | 0.1931 |
| Rwork | 0.192 |
| R-free | 0.21240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r3h |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.810 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.790 | 1.330 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Number of reflections | 88036 | 13842 |
| <I/σ(I)> | 11.71 | |
| Completeness [%] | 97.3 | |
| Redundancy | 4.55 | |
| CC(1/2) | 0.999 | 0.519 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25% PEG3350, 0.2M Ammonium Sulphate, 0.1M bis-tris, pH 6.5 |
