6ZD2

Structure of apo telomerase from Candida Tropicalis truncated from C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978910
Spacegroup name	C 2 2 21
Unit cell lengths	114.900, 154.850, 107.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.010 - 2.840
R-factor	0.2038
Rwork	0.202
R-free	0.23800
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.472
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.820	2.944
High resolution limit [Å]	2.840	2.842
Rmerge	0.078	0.625
Rmeas	0.084	0.677
Rpim		0.257
Number of reflections	22283	2280
<I/σ(I)>	17.32	2.53
Completeness [%]	96.9	99.56
Redundancy	6.6	6.8
CC(1/2)	0.997	0.916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	298	Tacsimate 60%