6ZCT

Nonstructural protein 10 (nsp10) from SARS CoV-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	I 21 3
Unit cell lengths	106.736, 106.736, 106.736
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.750 - 2.550
R-factor	0.1901
Rwork	0.190
R-free	0.19460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fyg
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.750	33.750	2.620
High resolution limit [Å]	2.550	11.400	2.550
Rmerge	0.114	0.037	0.949
Rmeas	0.124	0.041	1.026
Total number of observations	45822
Number of reflections	6757	87	500
<I/σ(I)>	11.42	34.8	1.72
Completeness [%]	100.0	95.6	100
Redundancy	6.781	5.368	6.99
CC(1/2)	0.997	0.998	0.737

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1 M Bis-Tris pH 5.5 3 M NaCl