6ZCT
Nonstructural protein 10 (nsp10) from SARS CoV-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.976 |
Spacegroup name | I 21 3 |
Unit cell lengths | 106.736, 106.736, 106.736 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.750 - 2.550 |
R-factor | 0.1901 |
Rwork | 0.190 |
R-free | 0.19460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fyg |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.750 | 33.750 | 2.620 |
High resolution limit [Å] | 2.550 | 11.400 | 2.550 |
Rmerge | 0.114 | 0.037 | 0.949 |
Rmeas | 0.124 | 0.041 | 1.026 |
Total number of observations | 45822 | ||
Number of reflections | 6757 | 87 | 500 |
<I/σ(I)> | 11.42 | 34.8 | 1.72 |
Completeness [%] | 100.0 | 95.6 | 100 |
Redundancy | 6.781 | 5.368 | 6.99 |
CC(1/2) | 0.997 | 0.998 | 0.737 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M Bis-Tris pH 5.5 3 M NaCl |