6ZCJ
14-3-3sigma in complex with SLP76pS376 phosphopeptide crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-28 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.69 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.098, 111.876, 62.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.400 - 1.530 |
| R-factor | 0.1692 |
| Rwork | 0.168 |
| R-free | 0.19020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mhr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.922 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1-3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.190 | 1.560 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.035 | 0.430 |
| Number of reflections | 43290 | 1922 |
| <I/σ(I)> | 20.1 | |
| Completeness [%] | 99.1 | |
| Redundancy | 5.8 | |
| CC(1/2) | 1.000 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277.15 | 95 mM Hepes pH 7.1-7.7, 24-29% PEG400, 190 mM CaCl2, Glycerol 5% |
