6ZC9
Structure of 14-3-3 gamma in complex with Nedd4-2 14-3-3 binding motif Ser448
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-19 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | H 3 |
| Unit cell lengths | 205.707, 205.707, 74.649 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.772 - 1.899 |
| R-factor | 0.212551383511 |
| Rwork | 0.212 |
| R-free | 0.23935 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b05 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.608 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.772 | 1.967 |
| High resolution limit [Å] | 1.899 | 1.899 |
| Rmeas | 0.051 | 1.038 |
| Number of reflections | 92751 | 9202 |
| <I/σ(I)> | 20.39 | |
| Completeness [%] | 99.8 | |
| Redundancy | 5.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | PEG 400, magnesium chloride, HEPES, hexafluoro-2-propanol |
