6ZBK

Crystal structure of the human complex between RPAP3 and TRBP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.033
Spacegroup name	P 21 21 2
Unit cell lengths	39.770, 158.130, 32.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.530 - 1.490
R-factor	0.1789
Rwork	0.177
R-free	0.21110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cgv 4wyq
RMSD bond length	0.021
RMSD bond angle	2.054
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.530	1.580
High resolution limit [Å]	1.490	1.490
Number of reflections	34245	5166
<I/σ(I)>	18.6	5.1
Completeness [%]	99.0	94.1
Redundancy	6.2	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	PEG 3,350 and TacsimateTM