6ZB9

Exo-beta-1,3-glucanase from moose rumen microbiome, wild type

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.072897
Spacegroup name	P 1 21 1
Unit cell lengths	55.580, 88.040, 70.810
Unit cell angles	90.00, 100.99, 90.00

Refinement procedure

Resolution	47.547 - 2.500
R-factor	0.2036
Rwork	0.197
R-free	0.27260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SAD-generated model
RMSD bond length	0.009
RMSD bond angle	1.029
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.550	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	44159	4164
<I/σ(I)>	5.8
Completeness [%]	96.8
Redundancy	3.4
CC(1/2)	0.983	0.359

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.1 M Tris-HCl, 0.2 M MgCl2, 30% PEG 4000