6Z9A

Fructo-oligosaccharide transporter BT 1762-63

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.92
Spacegroup name	C 2 2 21
Unit cell lengths	119.716, 234.623, 169.206
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	106.637 - 3.100
R-factor	0.2089
Rwork	0.206
R-free	0.26840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t3r
RMSD bond length	0.009
RMSD bond angle	1.240
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	169.210	3.040
High resolution limit [Å]	2.990	2.990
Rpim	0.130	0.830
Number of reflections	48529	2364
<I/σ(I)>	5	0.7
Completeness [%]	100.0
Redundancy	7.2
CC(1/2)	0.960	0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.15 mM sodium formate 0.1 M Hepes pH7-7.5 16-20% PEG3350