6Z91

Copper transporter OprC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-18
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	1.407337
Spacegroup name	C 2 2 21
Unit cell lengths	154.478, 195.030, 165.473
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.740 - 2.600
R-factor	0.2239
Rwork	0.222
R-free	0.26180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fok
RMSD bond length	0.008
RMSD bond angle	1.078
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	165.470	2.640
High resolution limit [Å]	2.600	2.600
Rpim	0.055
Number of reflections	76695	7519
<I/σ(I)>	7.56
Completeness [%]	99.9
Redundancy	13.5
CC(1/2)		0.644

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.2 M Choline chloride 0.1 M Tris 12-16 % w/v PEG 2000 MME