6Z8T

Copper transporter OprC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.40889
Spacegroup name	C 2 2 21
Unit cell lengths	155.521, 196.453, 165.878
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.410 - 2.860
R-factor	0.21
Rwork	0.207
R-free	0.27210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fok
RMSD bond length	0.009
RMSD bond angle	1.198
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.410	2.910
High resolution limit [Å]	2.860	2.860
Rpim	0.207
Number of reflections	57731	5635
<I/σ(I)>	4.18
Completeness [%]	98.2
Redundancy	13.14
CC(1/2)		0.284

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.5 M Potassium chloride 0.05 M HEPES 12-16 % v/v PEG 400