6Z8I

Fructo-oligosaccharide transporter BT 1762-63

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	115.709, 232.746, 171.252
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.626 - 2.620
R-factor	0.2204
Rwork	0.217
R-free	0.27490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t3r
RMSD bond length	0.009
RMSD bond angle	1.020
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.250	2.660
High resolution limit [Å]	2.620	2.620
Rpim	0.084	1.230
Number of reflections	69655	3318
<I/σ(I)>	7	0.8
Completeness [%]	99.9
Redundancy	7.3
CC(1/2)	0.995	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.15 M sodium formate 0.1 M Hepes pH 7-7.5 16-20% PEG3350